mouse anti human il 4 pe  (Miltenyi Biotec)

Journal: Cell Reports Medicine

Article Title: Safety and immunogenicity of SARS-CoV-2 self-amplifying RNA vaccine expressing an anchored RBD: A randomized, observer-blind phase 1 study

doi: 10.1016/j.xcrm.2023.101134

Figure Lengend Snippet: CD4 + T cell responses Flow cytometric analysis was performed to analyze RBD-specific T cells. Responses to VLPCOV-01, BNT162b2, or placebo are shown as fold change from baseline (day 1) to week 4 (day 29) for each cohort. The experiment was performed once. (A) The activated CD4 + T cells, characterized by the expression of CD154. (B) The response in CD4 + Th1 cells was characterized by the expression of interleukin-2, tumor necrosis factor α, and/or interferon-γ. (C) The response in CD4 + Th2 cells, which was measured by expression of interleukin-4 and/or -13. (D) The response in CD4 + Th17 cells, characterized by the expression of interleukin-17. (E) The CD4 + IL-21+ cells, which were measured by expression of IL-21. (F) The correlation between IgG antibody titers against SARS-CoV-2 RBD protein and the percentage of RBD-specific CD4 + Th1 cell responses following vaccination with VLPCOV-01. Spearman’s rank correlation coefficient and p value were calculated following log transformation of source data (r = 0.2717, p = 0.0092). (G–K) CD8 + cells expressing interferon-γ, tumor necrosis factor α, CD107a, macrophage inflammatory protein 1b, or interleukin-2, respectively. (A–E and G–K) The horizontal bars indicate geometric mean value.

Article Snippet: Mouse anti-human IL-4-PE-Cy7 (clone:8D4-8) , BD Biosciences , cat# 560672; RRID: AB_1727547.

Techniques: Expressing, Transformation Assay

Journal: Cell Reports Medicine

Article Title: Safety and immunogenicity of SARS-CoV-2 self-amplifying RNA vaccine expressing an anchored RBD: A randomized, observer-blind phase 1 study

doi: 10.1016/j.xcrm.2023.101134

Figure Lengend Snippet:

Article Snippet: Mouse anti-human IL-4-PE-Cy7 (clone:8D4-8) , BD Biosciences , cat# 560672; RRID: AB_1727547.

Techniques: Recombinant, Staining, Sequencing, Luciferase, Software

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Lymphostatin, a virulence factor of attaching and effacing Escherichia coli , inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis

doi: 10.3389/fcimb.2022.941939

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: PE Mouse Anti-Human IL-4 , 3010.211 , BD Biosciences.

Techniques: Purification, Western Blot

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Lymphostatin, a virulence factor of attaching and effacing Escherichia coli , inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis

doi: 10.3389/fcimb.2022.941939

Figure Lengend Snippet: Lymphostatin inhibits intracellular cytokine production in anti-CD3/anti-CD28 or ConA-stimulated CD4 + and CD8 + T cells. Dot plots show the percentage of CD4 + /CD69 + cells producing IL-2, IFN-γ, IL-4, IL-10 and CD8 + /CD69 + cells producing IL-2, IFN-γ in the presence of WT-rLifA, rLifA DTD-AAA , or carrier buffer. Data represent the mean from 8 healthy donors ± S.E.M. * p < 0.05, ** p < 0.01, *** p < 0.001 using Kruskal-Wallis test followed by Dunn’s test.

Article Snippet: PE Mouse Anti-Human IL-4 , 3010.211 , BD Biosciences.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Lymphostatin, a virulence factor of attaching and effacing Escherichia coli , inhibits proliferation and cytokine responses of human T cells in a manner associated with cell cycle arrest but not apoptosis or necrosis

doi: 10.3389/fcimb.2022.941939

Figure Lengend Snippet: Lymphostatin did not inhibit intracellular cytokine production in PMA/ionomycin-stimulated CD4 + and CD8 + T cells. Dot plots show the percentage of CD4 + /CD69 + cells producing IL-2, IFN-γ, IL-4, IL-10 and CD8 + /CD69 + cells producing IL-2, IFN-γ in the presence of WT-rLifA or left untreated (cells with mitogen; no tested protein) and compared between anti-CD3/anti-CD28 and PMA/ionomycin stimulation. Data represent the mean from 8 healthy donors ± S.E.M. * p < 0.05, ** p < 0.01, *** p < 0.001 using Kruskal-Wallis test followed by Dunn’s test.

Article Snippet: PE Mouse Anti-Human IL-4 , 3010.211 , BD Biosciences.

Techniques: